SkinAxis generates 3D skin models for the highly sensitive detection of UV damage using genetically engineered skin cells. Each cell type is engineered to allow the expression a reporter gene upon activation of the DNA damage response pathway. As UV irradiation causes DNA damage, these cells are particularly suitable to assess the effects of UV on skin in a quantifiable manner. Engineered cells are able to reconstitute the normal architecture of skin tissue after establishing air-liquid interface and cultivation in differentiation media. Novel 3D skin models allow rapid and quantitative assessment of the effects of UVA and UVB irradiation.
SkinAxis evaluates morphological changes after UVA or UVB exposure by H&E staining of skin equivalents and DNA damage by immohistochemical staining (e.g. thymidine dimers). Highly sensitive quantitative measurement of DNA damage by ELISA to determine cyclobutane pyrimidine dimers (CPD) is also available.
